Asian Pac J Cancer Prev. 2012;13(6):2533-9.
Chemopreventive potential of Annona muricata L leaves on chemically-induced skin papillomagenesis in mice.
Abstract
Annona
muricata L (Annonaceae), commonly known as soursop has a long, rich
history in herbal medicine with a lengthy recorded indigenous use. It
had also been found to be a promising new anti-tumor agent in numerous
in vitro studies. The present investigation concerns chemopreventive
effects in a two-stage model of skin papillomagenesis. Chemopreventive
effects of an ethanolic extract of A. muricata leaves (AMLE) was
evaluated in 6-7 week old ICR mice given a single topical application of
7,12-dimethylbenza(α)anthracene (DMBA 100 μg/100 μl acetone) and
promotion by repeated application of croton oil (1% in acetone/ twice a
week) for 10 weeks. Morphological tumor incidence, burden and volume
were measured, with histological evaluation of skin tissue. Topical
application of AMLE at 30, 100 and 300 mg/kg significantly reduced
DMBA/croton oil induced mice skin papillomagenesis in (i)
peri-initiation protocol (AMLE from 7 days prior to 7 days after DMBA),
(ii) promotion protocol (AMLE 30 minutes after croton oil), or (iii)
both peri-initiation and promotion protocol (AMLE 7 days prior to 7 day
after DMBA and AMLE 30 minutes after croton oil throughout the
experimental period), in a dose dependent manner (p<0.05) as compared
to carcinogen-treated control. Furthermore, the average latent period
was significantly increased in the AMLE-treated group. Interestingly, At
100 and 300 mg/ kg, AMLE completely inhibited the tumor development in
all stages. Histopathological study revealed that tumor growth from the
AMLE-treated groups showed only slight hyperplasia and absence of
keratin pearls and rete ridges. The results, thus suggest that the
A.muricata leaves extract was able to suppress tumor initiation as well
as tumor promotion even at lower dosage.
- PMID:
- 22938417
- [PubMed - indexed for MEDLINE]
