Volume 50, October 2013, Pages 88–94
Comparison of antioxidant, anti-inflammatory, antimicrobial activity and chemical composition of aqueous and hydroethanolic extracts of the herb of Tropaeolum majus L.
Highlights
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- The activity of tested extracts does not depend on how the plant material was prepared or how the extracts were prepared.
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- Aqueous and hydroethanolic extracts of nasturtium herb show strong scavenging activity against reactive nitrogen species.
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- The extracts show no antimicrobial activity.
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- The extracts show no inhibitory activity on hyaluronidase, but they inhibited activity of cyclooxygenase 1.
Abstract
The
aim of this study was comparison of antioxidant, anti-inflammatory, and
antimicrobial activity, as well as chemical composition of extracts of Tropaeolum majus
L. herb. Aqueous and hydroethanolic extracts derived from dried and
freeze-dried nasturtium herb, prepared both at room temperature and at
90 °C, were studied. In the studies there were no significant
differences between antioxidant activity of the extracts. All extracts
showed scavenging activity against all the examined reactive species in a
concentration-dependent manner. The strongest scavenging activity they
showed against reactive nitrogen species, NO
(SC50 4.54 ± 0.26–10.90 ± 1.39 μg/mL) and ONOO− (SC50 2.49 ± 1.50–6.37 ± 1.86 μg/mL). Among reactive oxygen species, they showed strong scavenging activity against H2O2 (SC50 14.90 ± 3.91–38.63 ± 9.28 μg/mL). Scavenging activity against O2−
was weaker, while against HClO the extracts showed very weak activity,
practically at the level of statistical error. Against synthetic radical
– DPPH scavenging activity of the tested extracts was negligible. The
extracts demonstrated stronger antioxidant activity in ex vivo
experiment on human neutrophils. The extracts showed no inhibitory
activity on hyaluronidase, but at a concentration of 50 μg/mL they
inhibited the activity of COX1 by approximately 60%. Lack of
antimicrobial activity of the extracts seems to be associated with a low
content of benzyl isothiocyanate. The aqueous extracts were
characterized by the presence of esters of quinic acid with cinnamic
acids (chlorogenic acids, p-coumaroylquinic acids) and the presence of
flavonoids. Meanwhile the hydroethanolic extracts were mainly rich in
the above mentioned acid esters.
(SC50 4.54 ± 0.26–10.90 ± 1.39 μg/mL) and ONOO− (SC50 2.49 ± 1.50–6.37 ± 1.86 μg/mL). Among reactive oxygen species, they showed strong scavenging activity against H2O2 (SC50 14.90 ± 3.91–38.63 ± 9.28 μg/mL). Scavenging activity against O2−
was weaker, while against HClO the extracts showed very weak activity,
practically at the level of statistical error. Against synthetic radical
– DPPH scavenging activity of the tested extracts was negligible. The
extracts demonstrated stronger antioxidant activity in ex vivo
experiment on human neutrophils. The extracts showed no inhibitory
activity on hyaluronidase, but at a concentration of 50 μg/mL they
inhibited the activity of COX1 by approximately 60%. Lack of
antimicrobial activity of the extracts seems to be associated with a low
content of benzyl isothiocyanate. The aqueous extracts were
characterized by the presence of esters of quinic acid with cinnamic
acids (chlorogenic acids, p-coumaroylquinic acids) and the presence of
flavonoids. Meanwhile the hydroethanolic extracts were mainly rich in
the above mentioned acid esters.Graphical abstract
Keywords
- Tropaeolum majus L.;
- Reactive oxygen/nitrogen species;
- Anti-inflammatory;
- Antimicrobial;
- HPLC–DAD–MSn
Copyright © 2013 Elsevier B.V. All rights reserved.
