South African Journal of Botany

Available online 15 August 2015

In Press, Corrected Proof — Note to users

Memory-vitalizing effect of twenty-five medicinal and edible plants and their isolated compounds

• F. Sezer Senol^a,
• Ilkay Erdogan Orhan^{a, ,} ,
• Ufuk Ozgen^b,
• Gulin Renda^b,
• Gokhan Bulut^c,
• Leyla Guven^c,
• Esen Sezen Karaoglan^c,
• Handan G. Sevindik^c,
• Krystyna Skalicka-Wozniak^d,
• Ufuk Koca Caliskan^a,
• Nazim Sekeroglu^e

doi:10.1016/j.sajb.2015.07.011

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Highlights

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The extracts from 25 plant species and 8 compounds were screened in vitro.

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Thymus sipyleus subsp. sipyleus var. sipyleus had the highest iron-chelating effect.

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Onosma nigricaule showed the most promising anti-AChE activity.

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The best anti-BChE was observed with Hypericum capitatum var. capitatum.

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The isolated compounds had none to moderate level of enzyme-inhibiting profiles.

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Abstract

The extracts from 25 plant species from Salvia, Hypericum, Onosma, Thymus, Origanum, Rosa, and Prunus with eight isolated compounds were screened for their memory-vitalizing potential against the enzymes, i.e., acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR) along with DPPH, DMPD, and NO radicals and iron-chelation capacity using spectrophotometric microtiter assays. The n-hexane:dichloromethane (1:1) extract of Onosma nigricaule roots (63.18 ± 0.56%) and the ethyl acetate extract of the aerial parts of Hypericum capitatum var. capitatum (50.79 ± 3.88%) were the most active toward AChE and BChE, respectively. The highest iron-chelating capacity was found in the n-hexane extract of Thymus sipyleus subsp. sipyleus var. sipyleus, where mostly Labiatae species exhibited better radical scavenging effect. Among the extracts, the roots of O. nigricaule, the aerial parts of Hypericum capitatum var. capitatum and T. sipyleus subsp. sipyleus var. sipyleus could be further evaluated for their memory-vitalizing properties based on different mechanisms.

Abbreviations

• ACh, acetylcholine;
• AChE, acetylcholinesterase;
• AD, Alzheimer's disease;
• ALS, amyotrophic lateral sclerosis;
• Aβ, amyloid beta;
• BChE, butyrylcholinesterase;
• DMPD, N,N-dimethyl-p-phenylendiamine;
• DMSO, dimethyl sulfoxide;
• DPPH, 2,2-diphenyl-1-picrylhydrazyl;
• DTNB, 5,5′-dithio-bis(2-nitrobenzoic)acid;
• EDTA, ethylenediaminetetraacetic acid;
• EtOAc, ethyl acetate;
• EtOH, ethanol;
• MeOH, methanol;
• NO, nitric oxide;
• PD, Parkinson's disease;
• SEM, standard error of the mean;
• TYR, tyrosinase

Keywords

• Cholinesterase;
• Tyrosinase;
• Enzyme inhibition;
• Antioxidant;
• Extract;
• Screening

Corresponding author. Tel.: + 90 312 2023186; fax: + 90 312 2235018.

Copyright © 2015 South African Association of Botanists. Published by Elsevier B.V. All rights reserved.

Note to users: Corrected proofs are Articles in Press that contain the authors' corrections. Final citation details, e.g., volume and/or issue number, publication year and page numbers, still need to be added and the text might change before final publication.