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Tuesday 27 October 2015

2012 Effect of in vitro digestion upon the antioxidant capacity of aqueous extracts of Agrimonia eupatoria, Rubus idaeus, Salvia sp. and Satureja montana

Volume 131, Issue 3, 1 April 2012, Pages 761-767

Effect of in vitro digestion upon the antioxidant capacity of aqueous extracts of Agrimonia eupatoria, Rubus idaeus, Salvia sp. and Satureja montana  (Article)

CBQF/Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, P-4200-072 Porto, Portugal
Department of Biochemistry (U38-FCT), Faculty of Medicine, University of Porto, Alameda Prof. Hernâni Monteiro, P-4200-319 Porto, Portugal
Department of Chemistry, Faculty of Sciences, University of Porto, Rua do Campo Alegre 687, P-4169-007 Porto, Portugal

Abstract

An in vitro digestion/Caco-2 cell culture model was used to assess the stability and bioavailability of phenolic compounds in aqueous extracts of four herbal infusions, traditionally used as functional drinks in Portugal. Alterations in antioxidant power were monitored by ABTS+, whereas the profile of phenolic compounds was ascertained by HPLC-DAD. The bioavailability of rutin - an important flavonoid present in such extracts, and thus a representative of those compounds, was assessed across Caco-2 cells via LC-MS/MS. Our results indicate that several antioxidant compounds are not affected by the (simulated) digestive process, whereas others undergo decreases in their activity throughout said process; a few have their antioxidant capacity hampered under stomach-like conditions. It was also found that rutin can be transported across the Caco-2 cell barrier. © 2011 Elsevier Ltd. All rights reserved.

Author keywords

Agrimony; Antioxidant profile; Caco-2 cells; Raspberry; Sage; Savory

Indexed keywords

Agrimony; Antioxidant profile; Caco-2 cells; Raspberry; Sage; Savory
Engineering controlled terms: Alcohols; Beverages; Biochemistry; Cell culture; Cells; Phenols
Engineering main heading: Solvent extraction
EMTREE drug terms: Agrimonia eupatoria extract; phenol derivative; plant extract; Rubus idaevus extract; rutoside; Salvia extract; Satureja montana extract; unclassified drug
EMTREE medical terms: Agrimonia; agrimonia eupatoria; antioxidant activity; article; cell strain CACO 2; controlled study; digestion; drug bioavailability; drug stability; drug transport; high performance liquid chromatography; in vitro study; liquid chromatography; mass spectrometry; rubus idaeus; Salvia; satureja montana; simulation
Species Index: Agrimonia; Agrimonia eupatoria; Rubus glaucus; Rubus idaeus; Salvia; Satureja montana
Chemicals and CAS Registry Numbers: rutoside, 153-18-4, 22519-99-9
ISSN: 03088146 CODEN: FOCHDSource Type: Journal Original language: English
DOI: 10.1016/j.foodchem.2011.09.030Document Type: Article