a
Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria
b Center for Physiology and Pharmacology, Medical University of Vienna, Schwarzspanierstr. 17, Vienna, Austria
c Department of Cellular and Molecular Physiology, Harris-Wellbeing Preterm Birth Centre, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom
b Center for Physiology and Pharmacology, Medical University of Vienna, Schwarzspanierstr. 17, Vienna, Austria
c Department of Cellular and Molecular Physiology, Harris-Wellbeing Preterm Birth Centre, Institute of Translational Medicine, University of Liverpool, Liverpool, United Kingdom
Abstract
Ethnopharmacological
relevance People living in the tropical rain forest of South-Western
Nigeria use Rinorea dentata (P. Beauv.) Kuntze (Violaceae) in
ethno-veterinary medicine to facilitate parturition. There are no
evidence-based pharmacological investigations for the uterotonic
activity of this plant. Aims of study (i) Collection of data about the
ethnopharmacological uses of R. dentata and evaluation of its uses and
applications in health care; (ii) determining potential uterotonic
effects in vitro, and (iii) chemical characterization of R. dentata,
which is a member of the Violaceae family known to express circular
cystine-knot peptides, called cyclotides. Materials and methods The
ethnopharmacological use of R. dentata in settlement camps within the
area J4 of Omo forest has been investigated by semi-structured
questionnaires and open interviews. Use index analysis has been
performed by seven quantitative statistical models. Respondents' claim
on the beneficial ethno-veterinary application of the plant to aid
parturition has been investigated in vitro by myometrial contractility
organ bath assays. The bioactive plant extract was screened by chemical
derivatization and mass spectrometry-based peptidomics using
reversed-phase HPLC fractionation and MALDI-TOF/TOF analysis. Results
Based on the survey analysis, medicinal preparations of R. dentata have
been used for anti-microbial and anti-malaria purpose in humans, and for
aiding parturition in farm animals. The latter application was
mentioned by one out of six respondents who claimed to use this plant
for any medicinal purpose. The plant extract exhibited a weak uterotonic
effect using organ bath studies. The plant contains cyclotides and the
peptide riden A has been identified by de novo amino acid sequencing
using mass spectrometry. Conclusion Few dwellers around the settlement
camps of the tropical forest of Omo (Nigeria) use R. dentata for various
health problems in traditional veterinary and human medicine. The weak
uterotonic effect of the cyclotide-rich extract is in agreement with the
low use value index obtained for this plant. Cyclotides have been
reported in the genus Rinorea confirming the ubiquitous expression of
these stable bioactive plant peptides within the family of Violaceae. ©
2015 The Authors.
Author keywords
Cyclotides; Ethnopharmacology; Rinorea dentata; Uterus muscle contractility; Veterinary medicine; Violaceae
Indexed keywords
EMTREE drug terms: antiinfective agent; antimalarial agent; cyclotide; plant extract; riden A; unclassified drug; uterotonic agent
EMTREE medical terms: Article; contraction assay;
ethnopharmacology; Nigerian; nonhuman; peptidomics; reversed phase high
performance liquid chromatography; Rinorea dentata; semi structured
interview; sequence analysis; traditional medicine; uterus
contractility; Violaceae
ISSN: 03788741
CODEN: JOETDSource Type: Journal
Original language: English
DOI: 10.1016/j.jep.2015.12.038Document Type: Article
Publisher: Elsevier Ireland Ltd
Funding Details
Number; Acronym; Sponsor: P24743-B11; FWF; Austrian Science Fund
