Effects of the Czech propolis on sperm mitochondrial function

Evidence-based Complementary and Alternative Medicine

Volume 2014, 2014, Article number 248768

Open Access

Cedikova, M.^ab , 

Miklikova, M.^ab , 

Stachova, L.^c , 

Grundmanova, M.^d , 

Tuma, Z.^be , 

Vetvicka, V.^f , 

Zech, N.^g , 

Kralickova, M.^ab , 

Kuncova, J.^bd 

 

^a  Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University in Prague, 301 00 Pilsen, Czech Republic
^b  Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, 301 00 Pilsen, Czech Republic
^c  Institute of Pharmacology and Toxicology, Faculty of Medicine in Pilsen, Charles University in Prague, 301 00 Pilsen, Czech Republic 

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Abstract

Propolis is a natural product that honeybees collect from various plants. It is known for its beneficial pharmacological effects. The aim of our study was to evaluate the impact of propolis on human sperm motility, mitochondrial respiratory activity, and membrane potential. Semen samples from 10 normozoospermic donors were processed according to the World Health Organization criteria. Propolis effects on the sperm motility and mitochondrial activity parameters were tested in the fresh ejaculate and purified spermatozoa. Propolis preserved progressive motility of spermatozoa in the native semen samples. Oxygen consumption determined in purified permeabilized spermatozoa by high-resolution respirometry in the presence of adenosine diphosphate and substrates of complex I and complex II (state OXPHOS I + II) was significantly increased in the propolis-treated samples. Propolis also increased uncoupled respiration in the presence of rotenone (state ETS II) and complex IV activity, but it did not influence state LEAK induced by oligomycin. Mitochondrial membrane potential was not affected by propolis. This study demonstrates that propolis maintains sperm motility in the native ejaculates and increases activities of mitochondrial respiratory complexes II and IV without affecting mitochondrial membrane potential. The data suggest that propolis improves the total mitochondrial respiratory efficiency in the human spermatozoa in vitro thereby having potential to improve sperm motility. © 2014 Miroslava Cedikova et al.

Indexed keywords

EMTREE drug terms: adenosine diphosphate; cytochrome c oxidase; propolis; reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone); rotenone; succinate dehydrogenase (ubiquinone)

EMTREE medical terms: adult; article; asthenospermia; cell permeabilization; dose response; drug effect; ejaculation; high performance liquid chromatography; human; male; mitochondrial membrane potential; nonhuman; oxygen consumption; respirometry; semen analysis; separation technique; spermatozoon; spermatozoon density; spermatozoon motility

Chemicals and CAS Registry Numbers: adenosine diphosphate, 20398-34-9, 58-64-0; cytochrome c oxidase, 72841-18-0, 9001-16-5; propolis, 8012-89-3; reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone), 9028-04-0; rotenone, 83-79-4; succinate dehydrogenase (ubiquinone), 9028-11-9

ISSN: 1741427XSource Type: Journal Original language: English

DOI: 10.1155/2014/248768Document Type: Article

Publisher: Hindawi Publishing Corporation

References (42)

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  Kuncova, J.; Biomedical Centre, Faculty of Medicine in Pilsen, Charles University in Prague, 301 00 Pilsen, Czech Republic; email:jitka.kuncova@lfp.cuni.cz
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