- 1Research
and Development Functional Food Centre (CIDAF), Bioregión Building,
Health Science Technological Park, Avenida del Conocimiento s/n, 18016,
Granada, Spain.
- 2Laboratoire d'Electrochimie et
Environnement, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax,
BP "1173", 3038, Tunisia.
- 3Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, 18071, Granada, Spain.
- 4Department of Analytical Chemistry, University of Cordoba, Annex C-3 Building, Campus of Rabanales, 14071, Córdoba, Spain.
Abstract
INTRODUCTION:
Olea europaea
L. organs such as leaves, stems and roots have been associated with
numerous in vivo and in vitro biological activities and used for
traditional medicinal purposes. However, tree wood is an untapped
resource with little information about their chemical composition.
OBJECTIVE:
That
is why, the objective of this study is to increase the knowledge about
phytochemicals from 'Chemlali' olive wood by means of mass
spectrometry-based analyses. Its comparison with by-products derived
from leaves was also studied.
METHODOLOGY:
Hydromethanol
extracts from wood and leaves with stems of 'Chemlali' olive cultivar
were analysed using reversed-phase (RP) high-performance liquid
chromatography (HPLC) coupled to two detection systems: diode-array
detection (DAD) and quadrupole time-of-flight (QTOF) mass spectrometry
(MS) in negative ion mode. Tandem MS experiments were performed to
establish the chemical structure of olive phytochemicals.
RESULTS:
A
total of 85 compounds were characterised in the studied olive parts and
classified as: sugars (3), organic acids (5), one phenolic aldehyde,
simple phenolic acids (6), simple phenylethanoids (5), flavonoids (14),
coumarins (3), caffeoyl phenylethanoid derivatives (6), iridoids (5),
secoiridoids (32), and lignans (5). To our knowledge, the major part of
these metabolites was not previously reported in olive tree wood, and 10
olive chemical constituents were identified for the first time in the
Oleaceae family.
CONCLUSION:
The
results presented here demonstrated the usefulness of the methodology
proposed, based on RP-HPLC-DAD-ESI-QTOF-MS and MS/MS, to develop an
exhaustive metabolic profiling and to recover new biologically active
compounds in olive wood with pharmacologic and cosmetic potential.
Copyright © 2017 John Wiley & Sons, Ltd.
Copyright © 2017 John Wiley & Sons, Ltd.
KEYWORDS:
olive leaves; olive wood; phytochemicals; secoiridoids; tandem mass spectrometry
